Article
Molecular dissection of the large mechanosensitive ion channel (MscL) of E. coli: mutants with altered channel gating and pressure sensitivity.
The Journal of membrane biology - 1 May 1997
Häse C C, Le Dain A C, Martinac B
Abstract excerpt
In the search for the essential functional domains of the large mechanosensitive ion channel (MscL) of E. coli, we have cloned several mutants of the mscL gene into a glutathione S-transferase fusion protein expression system. The resulting mutated MscL proteins had either amino acid additions, s...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- Base Sequence
- Escherichia coli
- Escherichia coli Proteins
- Ion Channel Gating
- Ion Channels
- Molecular Sequence Data
- Mutation
- Patch-Clamp Techniques