Article
An altered invariant chain protein with an antigenic peptide in place of CLIP forms SDS-stable complexes with class II alphabeta dimers and facilitates highly efficient peptide loading.
International immunology - 1 Aug 1998
Barton G M, Rudensky A Y
Abstract excerpt
We report an experimental system for abundant expression of specific peptide-class II complexes in vivo and in vitro. We have constructed a cassette which allows for the replacement of the CLIP region of invariant chain (Ii) with an antigenic peptide. In fibroblasts expressing an altered Ii prote...
Topics
- Amino Acid Sequence
- Animals
- Antigen Presentation
- Antigens, Differentiation, B-Lymphocyte
- Blotting, Western
- Dimerization
- Endoplasmic Reticulum, Rough
- Flow Cytometry
- Histocompatibility Antigens Class II
- Humans
- L Cells
- Membrane Proteins
- Mice
- Molecular Chaperones
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Peptide Fragments