Introduction of hereditary disease-associated mutations into the beta-amyloid precursor protein gene of mouse embryonic stem cells: a comparison of homologous recombination methods.

Abstract excerpt

Two different approaches for introducing pathogenic mutations into the beta-amyloid precursor protein gene in mouse embryonic stem cells were compared. Both approaches require two sequential modifications of the targeting locus by homologous recombinations. One approach was a "targeting-in-out" p...

Topics

• Alzheimer Disease
• Amyloid beta-Protein Precursor
• Animals
• Cells, Cultured
• Gene Targeting
• Humans
• Mice
• Mutation
• Stem Cells