Introduction of hereditary disease-associated mutations into the beta-amyloid precursor protein gene of mouse embryonic stem cells: a comparison of homologous recombination methods.

Abstract excerpt

Two different approaches for introducing pathogenic mutations into the beta-amyloid precursor protein gene in mouse embryonic stem cells were compared. Both approaches require two sequential modifications of the targeting locus by homologous recombinations. One approach was a "targeting-in-out" procedure that is based on a double-replacement strategy, and the other was a "hit-and-run" procedure that makes use of...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Alzheimer Disease
• Share to Amyloid beta-Protein Precursor