Article
Utilization of microhomologous recombination in yeast to generate targeting constructs for mammalian genes.
Mutation research - 5 Jun 1998
Khrebtukova I, Michaud E J, Foster C M, Stark K L, Garfinkel D J, Woychik R P
Abstract excerpt
We have developed a new procedure utilizing microhomologous recombination in yeast to generate targeting constructs for producing targeted mutations in mice. This procedure is rapid and efficient, and should be directly applicable to all mammalian genes. Moreover, only minimal information about the locus being targeted is required. The feasibility of this approach was demonstrated by producing another allele of...
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