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Article

Utilization of microhomologous recombination in yeast to generate targeting constructs for mammalian genes.

Mutation research - 5 Jun 1998

Khrebtukova I, Michaud E J, Foster C M, Stark K L, Garfinkel D J, Woychik R P

Abstract excerpt

We have developed a new procedure utilizing microhomologous recombination in yeast to generate targeting constructs for producing targeted mutations in mice. This procedure is rapid and efficient, and should be directly applicable to all mammalian genes. Moreover, only minimal information about t...

Topics

Alleles
Animals
Base Sequence
Chimera
Cloning, Molecular
DNA Primers
Exons
Gene Targeting
Genetic Vectors
Genomic Library
Mammals
Mice
Mice, Inbred Strains

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Molecular Sequence Data

Mutagenesis

Polycystic Kidney Diseases

Polymerase Chain Reaction

Recombination, Genetic

Source

Identifiers

PMID: 9639665
DOI: 10.1016/s0027-5107(98)00053-0

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9639665-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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