Article
A broad-host-range Flp-FRT recombination system for site-specific excision of chromosomally-located DNA sequences: application for isolation of unmarked Pseudomonas aeruginosa mutants.
Gene - 28 May 1998
Hoang T T, Karkhoff-Schweizer R R, Kutchma A J, Schweizer H P
Abstract excerpt
An improved method for gene replacement in Pseudomonas aeruginosa was developed. The method employs several new gene replacement vectors that incorporate (1) the counterselectable sacB marker, (2) a lacZ alpha-allele for blue-white screening, (3) the pUC18/19 vectors multiple cloning site with 10...
Topics
- Base Sequence
- Chromosome Mapping
- DNA Nucleotidyltransferases
- DNA Primers
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genetic Markers
- Genetic Techniques
- Genetic Vectors
- Molecular Sequence Data