Article
A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination.
BMC microbiology - 17 Apr 2013
Ishikawa Masahito, Hori Katsutoshi
Abstract excerpt
BACKGROUND: For the disruption of a target gene in molecular microbiology, unmarked mutagenesis is preferable to marked mutagenesis because the former method raises no concern about the polar effect and leaves no selection marker. In contrast to naturally competent bacteria, there is no useful method for introducing an unmarked mutation into a large gene of non-competent bacteria. Nevertheless, large genes...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.