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Article

Use of the lambda Red recombinase system to rapidly generate mutants in Pseudomonas aeruginosa.

BMC molecular biology - 4 Feb 2008

Lesic Biliana, Rahme Laurence G

Abstract excerpt

BACKGROUND: The Red recombinase system of bacteriophage lambda has been used to inactivate chromosomal genes in various bacteria and fungi. The procedure consists of electroporating a polymerase chain reaction (PCR) fragment that was obtained with a 1- or 3-step PCR protocol and that carries an antibiotic cassette flanked by a region homologous to the target locus into a strain that expresses the lambda Red...

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Identifiers

PMID: 18248677
DOI: 10.1186/1471-2199-9-20
PMCID: PMC2287187

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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