Article
Allelic exchange in Pseudomonas aeruginosa using novel ColE1-type vectors and a family of cassettes containing a portable oriT and the counter-selectable Bacillus subtilis sacB marker.
Molecular microbiology - 1 May 1992
Schweizer H P
Abstract excerpt
An improved method for allele replacement in Pseudomonas aeruginosa was developed. The two main ingredients of the method are: (i) novel ColE1-type cloning vectors derived from pBR322 and pUC19; and (ii) a family of cassettes containing a portable oriT, the sacB gene from Bacillus subtilis as a c...
Topics
- Alleles
- Amino Acid Sequence
- Bacillus subtilis
- Base Sequence
- DNA, Recombinant
- Escherichia coli
- Genetic Markers
- Genetic Vectors
- Molecular Sequence Data
- Mutagenesis, Insertional
- Plasmids
- Pseudomonas aeruginosa
- Transduction, Genetic