Article
CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida.
Biotechnology journal - 1 May 2018
Aparicio Tomás, de Lorenzo Víctor, Martínez-García Esteban
Abstract excerpt
While adoption of single-stranded DNA recombineering techniques has greatly eased genetic design of the platform strain Pseudomonas putida KT2440, available methods still produce the desired modifications/deletions at low frequencies. This makes isolation of mutants that do not display selectable or conspicuous phenotypes considerably difficult. To overcome this limitation, the authors have merged ssDNA...
Topics
- CRISPR-Cas Systems
- Gene Editing
- Mutation
- Plasmids
- Pseudomonas putida