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Article

Site-directed mutagenesis and gene deletion using reverse genetics.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Muhl Daniela, Filloux Alain

Abstract excerpt

Understanding gene function is far easier when tools are available to engineer a bacterial strain lacking a specific gene and phenotypically compare its behavior with the corresponding parental strain. Such mutants could be selected randomly, either by natural selection under particular stress conditions or by random mutagenesis using transposon delivery as described elsewhere in this book. However, with the...

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Share to Deoxyribonucleases, Type II Site-Specific
Share to Gene Amplification
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Mutagenesis, Site-Directed

Share to Pseudomonas aeruginosa

Share to Recombination, Genetic

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Identifiers

PMID: 24818930
DOI: 10.1007/978-1-4939-0473-0_40

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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