Alterations of TP53 in microdissected transitional cell carcinoma of the human urinary bladder: high frequency of TP53 accumulation in the absence of detected mutations is associated with poor prognosis.

Abstract excerpt

We have used microdissection of paraffin-embedded histological sections and polymerase chain reaction (PCR)-based direct DNA sequencing for 54 transitional cell carcinoma (TCC) of the bladder, to examine critically the association between TP53 nuclear accumulation determined by immunohistochemistry and the presence of TP53 mutations, and to examine their relationship to tumour stage and grade, as well as patient...

Topics

• Carcinoma, Transitional Cell
• DNA, Neoplasm
• Dissection
• Exons
• Genes, p53
• Humans
• Immunohistochemistry
• Mutation
• Paraffin Embedding
• Polymerase Chain Reaction