Article
mRNA and protein expression of p53 mutations in human bladder cancer cell lines.
Cancer letters - 15 Jul 1994
Kawasaki T, Tomita Y, Watanabe R, Tanikawa T, Kumanishi T, Sato S
Abstract excerpt
We investigated mRNA and protein expression in p53 gene mutations in four human bladder cancer cell lines using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and Northern blot and Western blot analyses. The following mutations were identified in three of the four cell lines: a missense transversion at codon 110, a missense transition at codon 250 and a non-sense transversion at...
Topics
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Genes, p53
- Humans
- Immunohistochemistry
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- RNA, Messenger
- Tumor Cells, Cultured