Article
Transcription-associated mutagenesis in yeast is directly proportional to the level of gene expression and influenced by the direction of DNA replication.
DNA repair - 1 Sept 2007
Kim Nayun, Abdulovic Amy L, Gealy Regan, Lippert Malcolm J, Jinks-Robertson Sue
Abstract excerpt
A high level of transcription has been associated with elevated spontaneous mutation and recombination rates in eukaryotic organisms. To determine whether the transcription level is directly correlated with the degree of genomic instability, we have developed a tetracycline-regulated LYS2 reporter system to modulate the transcription level over a broad range in Saccharomyces cerevisiae. We find that spontaneous...
Topics
- Base Sequence
- DNA Repair
- DNA Replication
- DNA, Fungal
- Gene Expression Regulation, Fungal
- Genomic Instability
- Molecular Sequence Data
- Mutagenesis
- Mutation
- Reverse Transcriptase Polymerase Chain Reaction
- Saccharomyces cerevisiae
- Saccharomyces cerevisiae Proteins