Article
Specific RecA amino acid changes affect RecA-UmuD'C interaction.
Molecular microbiology - 1 Apr 1998
Sommer S, Boudsocq F, Devoret R, Bailone A
Abstract excerpt
The UmuD'C mutagenesis complex accumulates slowly and parsimoniously after a 12 Jm(-2) UV flash to attain after 45 min a low cell concentration between 15 and 60 complexes. Meanwhile, RecA monomers go up to 72,000 monomers. By contrast, when the UmuD'C complex is constitutively produced at a high...
Topics
- Amino Acid Sequence
- Bacterial Proteins
- DNA-Directed DNA Polymerase
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli Proteins
- Immunoblotting
- Mitomycin
- Mutagenesis
- Mutation
- Rec A Recombinases
- SOS Response, Genetics
- Time Factors
- Ultraviolet Rays