Article
High expression in bacteria and purification of polymorphic mouse interleukin 2 molecules.
Cytokine - 1 Apr 1998
Matesanz F, Alcina A
Abstract excerpt
Recently, besides the known mouse interleukin 2 (IL-2) molecule, six other IL-2 alleles have been found in different mouse strains. In order to study their in vivo and in vitro biological activities large quantities are required. We cloned the corresponding IL-2 cDNAs into a pET7-7/BL21(DE3) bacterial system, a T7-RNA-polymerase-dependent expression vector, producing between 30 to 100 mg of IL-2 per litre of...
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