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Expression and purification of a mutant of human interleukin-2 in Pichia pastoris.

Applied biochemistry and biotechnology - 1 Apr 2006

Liu Yan, Xiao Xun-Yan, Sun Min, Hu Ying-He, Ou-Yang Ke-Qing, Cai Shao-Xi, Hua Zi-Chun

Abstract excerpt

Interleukin (IL)-2 is a pharmacologically important cytokine secreted by T-lymphocytes. Recombinant IL-2 (rIL-2) has been modified and produced in many systems. Mass production of rIL-2 is the prerequisite for its wide application. Using a site-directed mutagenesis strategy, we first generated a gene coding for a new type of mutant of human IL-2 (MhIL-2), in which we replaced the cysteine-125 in human IL-2 with...

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PMID: 16622285
DOI: 10.1385/abab:133:1:77

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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