Article
Purification of the IL-2 receptor (TAC) by ligand-affinity chromatography and utilization of the immobilized receptor for receptor-affinity chromatography (RAC) purification of IL-2, mutant IL-2, and IL-2 fusion proteins.
The Journal of investigative dermatology - 1 Jun 1990
Smart J E, Familletti P C, Weber D V, Keeney R F, Bailon P
Abstract excerpt
Recombinant technology has facilitated the production of two soluble forms of human p55 interleukin-2 receptor (IL-2R) in Chinese hamster ovary cells. We have developed a ligand-affinity method for the medium-scale purification of these two soluble forms of the IL-2R, based on the biochemical int...
Topics
- ADP Ribose Transferases
- Animals
- Bacterial Toxins
- Chromatography, Affinity
- Chromatography, Gel
- Cricetinae
- Cricetulus
- Exotoxins
- Female
- Interleukin-2
- Mutation
- Ovary
- Receptors, Interleukin-2
- Recombinant Fusion Proteins