Article
[Effective expression of genes for interleukin-2 and its mutant analogs in E. coli cells].
Molekuliarnaia biologiia - 1 Jan 2000
Seregin S V, Daniliuk N K, Siniakov A N, Kamynina T P, Il'iukova L V, Sakhno L V
Abstract excerpt
Recombinant plasmids were constructed for the efficient expression in E. coli cells of the human interleukin-2 (HIL-2) gene and two its mutant analogues obtained by of chemical-enzymic synthesis and polymerase chain reaction (deletion of 14 C-terminal amino acids and a change of the codon for Trp121 to Phe). The recombinant HIL-2 but not the mutant analogues were shown to be biologically active. Both analogues...
Topics
- Base Sequence
- Escherichia coli
- Gene Expression
- Genes, Synthetic
- Humans
- Interleukin-2
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Plasmids
- Recombinant Proteins