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Article

Application of a Bioassay with CHO Cells for the Routine Detection of Stimulating and Blocking Autoantibodies to the TSH-Receptor

1 Mar 1998

Abstract excerpt

The importance of bioassays measuring stimulating and blocking autoantibodies to the TSH-receptor (TSH-R) by their effect on cAMP production in CHO cells transfected with the recombinant TSH-R is increasingly recognized. The standard technique for this bioassay is cumbersome, as it involves purification of serum IgG with polyethylene glycol (PEG) and resuspension in hypotonic buffer. We have therefore established...

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PMID: 9566861
DOI: 10.1055/s-2007-978858

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