Article
Identification of separate determinants on the thyrotropin receptor reactive with Graves' thyroid-stimulating antibodies and with thyroid-stimulating blocking antibodies in idiopathic myxedema: these determinants have no homologous sequence on gonadotropin receptors.
Molecular endocrinology (Baltimore, Md.) - 1 Feb 1992
Kosugi S, Ban T, Akamizu T, Kohn L D
Abstract excerpt
Deletions, substitutions, or mutations of the rat TSH receptor extracellular domain between residues 20 and 107 (all residue numbers are determined by counting from the methionine start site) have been made by site-directed mutagenesis of receptor cDNA. After transfection in Cos-7 cells, constructs were evaluated for their ability to bind [125I]TSH or respond to TSH and thyroid-stimulating antibodies (TSAbs) from...
Topics
- Alanine
- Amino Acid Sequence
- Animals
- Asparagine
- Autoantibodies
- Binding Sites
- Binding, Competitive
- Cyclic AMP
- Graves Disease
- Humans