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Article

A single side chain prevents Escherichia coli DNA polymerase I (Klenow fragment) from incorporating ribonucleotides.

Proceedings of the National Academy of Sciences of the United States of America - 31 Mar 1998

Astatke M, Ng K, Grindley N D, Joyce C M

Abstract excerpt

Although nucleic acid polymerases from different families show striking similarities in structure, they maintain stringent specificity for the sugar structure of the incoming nucleoside triphosphate. The Klenow fragment of E. coli DNA polymerase I selects its natural substrates, deoxynucleotides, over ribonucleotides by several thousand fold. Analysis of mutant Klenow fragment derivatives indicates that...

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Identifiers

PMID: 9520378
DOI: 10.1073/pnas.95.7.3402
PMCID: PMC19848

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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