Article
A mutant of DNA polymerase I (Klenow fragment) with reduced fidelity.
Biochemistry - 22 Jan 1991
Carroll S S, Cowart M, Benkovic S J
Abstract excerpt
The kinetic parameters governing incorporation of correct and incorrect bases into synthetic DNA duplexes have been investigated for Escherichia coli DNA polymerase I [Klenow fragment (KF)] and for two mutants, Tyr766Ser and Tyr766Phe. Tyr766 is located at the C-terminus of helix O in the DNA-binding cleft of KF. The catalytic efficiency for correct incorporation of dNTP is reduced 5-fold for Tyr766Ser. The...
Topics
- Base Sequence
- Computer Simulation
- DNA
- DNA Polymerase I
- DNA Replication
- Escherichia coli
- Kinetics
- Molecular Sequence Data
- Mutation
- Phenylalanine
- Serine
- Tyrosine