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Article

A mutant of DNA polymerase I (Klenow fragment) with reduced fidelity.

Biochemistry - 22 Jan 1991

Carroll S S, Cowart M, Benkovic S J

Abstract excerpt

The kinetic parameters governing incorporation of correct and incorrect bases into synthetic DNA duplexes have been investigated for Escherichia coli DNA polymerase I [Klenow fragment (KF)] and for two mutants, Tyr766Ser and Tyr766Phe. Tyr766 is located at the C-terminus of helix O in the DNA-binding cleft of KF. The catalytic efficiency for correct incorporation of dNTP is reduced 5-fold for Tyr766Ser. The...

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PMID: 1899034
DOI: 10.1021/bi00217a034

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