Article
A conservative isoleucine to leucine mutation causes major rearrangements and cold sensitivity in KlenTaq1 DNA polymerase.
Biochemistry - 27 Jan 2015
Wu Eugene Y, Walsh Amanda R, Materne Emma C, Hiltner Emily P, Zielinski Bryan, Miller Bill R, Mawby Lily, Modeste Erica, Parish Carol A, Barnes Wayne M, Kermekchiev Milko B
Abstract excerpt
Assembly of polymerase chain reactions at room temperature can sometimes lead to low yields or unintentional products due to mispriming. Mutation of isoleucine 707 to leucine in DNA polymerase I from Thermus aquaticus substantially decreases its activity at room temperature without compromising its ability to amplify DNA. To understand why a conservative change to the enzyme over 20 Å from the active site can...
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