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Article

Enhanced ribonucleotide incorporation by an O-helix mutant of Thermus aquaticus DNA polymerase I.

Mutation research - 4 Apr 2001

Ogawa M, Tosaka A, Ito Y, Yoshida S, Suzuki M

Abstract excerpt

The O-helix of DNA polymerases has been implicated in substrate discrimination and replication fidelity. In this study, wild-type Thermus aquaticus DNA polymerase I (Taq pol I) and an O-helix mutant A661E was examined for their ability to discriminate between ribonucleotides and deoxyribonucleotides. Steady-state nucleotide extension kinetics were carried out using a template cytidine and each nucleotide dNTP and...

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Identifiers

PMID: 11267831
DOI: 10.1016/s0921-8777(00)00081-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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