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Article

Removal of misincorporated ribonucleotides from prokaryotic genomes: an unexpected role for nucleotide excision repair.

PLoS genetics - 1 Nov 2013

Vaisman Alexandra, McDonald John P, Huston Donald, Kuban Wojciech, Liu Lili, Van Houten Bennett, Woodgate Roger

Abstract excerpt

Stringent steric exclusion mechanisms limit the misincorporation of ribonucleotides by high-fidelity DNA polymerases into genomic DNA. In contrast, low-fidelity Escherichia coli DNA polymerase V (pol V) has relatively poor sugar discrimination and frequently misincorporates ribonucleotides. Substitution of a steric gate tyrosine residue with alanine (umuC_Y11A) reduces sugar selectivity further and allows pol V...

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Identifiers

PMID: 24244177
DOI: 10.1371/journal.pgen.1003878
PMCID: PMC3820734

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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