Modification of ricin A chain, by addition of endoplasmic reticulum (KDEL) or Golgi (YQRL) retention sequences, enhances its cytotoxicity and translocation.

doi:10.1007/s002620050460

Back to search

Article

Modification of ricin A chain, by addition of endoplasmic reticulum (KDEL) or Golgi (YQRL) retention sequences, enhances its cytotoxicity and translocation.

Cancer immunology, immunotherapy : CII - 1 Mar 1998

Zhan J, Stayton P, Press O W

Abstract excerpt

A pKK expression system in Escherichia coli was used to produce recombinant ricin A chain (rRTA) and rRTA modified by addition of organelle-specific amino acid retention sequences, including KDEL (an endoplasmic reticulum, ER, lumen retention signal), KKMP (an ER membrane retention signal), YQRL...

Topics

Cytosol
Endoplasmic Reticulum
Escherichia coli
Golgi Apparatus
Humans
Jurkat Cells
Mutation
Recombinant Proteins
Ricin

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.