Article
Calcium binding to the first EGF-like module of human factor IX in a recombinant fragment containing residues 1-85. Mutations V46E and Q50E each manifest a negligible increase in calcium affinity.
FEBS letters - 9 Jan 1998
Persson K E, Astermark J, Björk I, Stenflo J
Abstract excerpt
The first EGF-like module of human coagulation factor IX contains a single functionally important calcium ion binding site. We have now shown the dissociation constant for this site to be approximately 160 microM in a recombinant protein fragment consisting of residues 1-85 in human fIX. This rep...
Topics
- Amino Acid Sequence
- Animals
- Calcium
- Cell Line
- Epidermal Growth Factor
- Factor IX
- Glutamic Acid
- Glutamine
- Humans
- Molecular Sequence Data
- Mutation
- Recombinant Fusion Proteins
- Spodoptera
- Valine