Article
Asp-70-->Lys mutant of factor X lacks high affinity Ca2+ binding site yet retains function.
The Journal of biological chemistry - 26 Aug 1994
Rezaie A R, Esmon C T
Abstract excerpt
To study the role of a putative high affinity Ca2+ binding site in the protease domain of factor X, we prepared a deletion mutant (E2FX) lacking the Gla and first epidermal growth factor-like domains. E2FX possesses a single high affinity Ca2+ binding site (Kd = 154 microM). Asp-70 or Glu-80 (chymotrypsin numbering system) was then converted to Lys. These mutants, E2FXD70K and E2FXE80K, allow examination of the...
Topics
- Aspartic Acid
- Base Sequence
- Binding Sites
- Calcium
- Dose-Response Relationship, Drug
- Factor VIIa
- Factor X
- Humans
- Lysine
- Molecular Sequence Data
- Mutation
- Protein Binding