Article
The high affinity calcium-binding site involved in protein C activation is outside the first epidermal growth factor homology domain.
The Journal of biological chemistry - 15 Jun 1992
Rezaie A R, Esmon N L, Esmon C T
Abstract excerpt
Binding Ca2+ to a high affinity site in protein C and 4-carboxyglutamic acid (Gla)-domainless protein C results in a conformational change that is required for activation by the thrombin-thrombomodulin complex, the natural activator of protein C. It has been hypothesized that this high affinity C...
Topics
- 1-Carboxyglutamic Acid
- Base Sequence
- Binding Sites
- Calcium
- Cations, Divalent
- Cells, Cultured
- Electrophoresis, Polyacrylamide Gel
- Enzyme Activation
- Epidermal Growth Factor
- Humans
- Molecular Sequence Data
- Mutation
- Plasmids
- Protein C
- Protein Conformation
- Sequence Homology, Nucleic Acid
- Spectrometry, Fluorescence