Article
Characterization of the functional defect in factor IX Alabama. Evidence for a conformational change due to high affinity calcium binding in the first epidermal growth factor domain.
The Journal of biological chemistry - 25 Jun 1990
McCord D M, Monroe D M, Smith K J, Roberts H R
Abstract excerpt
Factor IX Alabama is a factor IX variant in which a glycine has been substituted for Asp47 in the first epidermal growth factor (EGF) domain. The structural defect in factor IX Alabama results in a molecule with 10% of normal coagulant activity. The interactions of immunoaffinity-purified factor...
Topics
- Binding Sites
- Calcium
- Computer Simulation
- Epidermal Growth Factor
- Factor IX
- Factor IXa
- Genetic Variation
- Humans
- Kinetics
- Models, Molecular
- Polylysine
- Protein Binding
- Protein Conformation
- Spectrometry, Fluorescence