Differential replication timing of X-linked genes measured by a novel method using single-nucleotide primer extension.

doi:10.1093/nar/26.2.684

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Article

Differential replication timing of X-linked genes measured by a novel method using single-nucleotide primer extension.

Nucleic acids research - 15 Jan 1998

Xiong Z, Tsark W, Singer-Sam J, Riggs A D

Abstract excerpt

The ratio of two differentially replicating alleles is not constant during S phase. Using this fact, we have developed a method for determining allele-specific replication timing for alleles differing by at least a single base pair. Unsynchronized cells in tissue culture are first sorted into fra...

Topics

Alleles
Animals
Base Composition
Cells, Cultured
DNA
DNA Primers
DNA Replication
Mice
Mice, Inbred C57BL
Polymerase Chain Reaction
RNA, Long Noncoding
RNA, Untranslated
Transcription Factors
X Chromosome

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