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Article

Factor V Leiden: detection in whole blood by ASA PCR using an additional mismatch in antepenultimate position.

Thrombosis research - 1 Oct 1997

Hézard N, Cornillet P, Droullé C, Gillot L, Potron G, Nguyen P

Abstract excerpt

Factor V Leiden mutation was initially detected in thrombophilic patients and relatives by PCR RFLP (Restriction Fragment Length Polymorphism) according to Bertina (1). This technique presents some drawbacks and the current trend is to simplify the diagnosis. We describe a technique of Allele Specific Amplification (ASA) which is optimized in terms of reliability: an additional mismatch in antepenultimate...

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Identifiers

PMID: 9336874
DOI: 10.1016/s0049-3848(97)00191-6

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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