Article
Simple and reliable factor V genotyping by PNA-mediated PCR clamping.
Thrombosis and haemostasis - 1 Apr 1998
Behn M, Schuermann M
Abstract excerpt
Resistance to activated protein C (APC resistance) is the most common cause of thrombophilia and linked to a single point mutation in the factor V gene (G-->A transition at nucleotide 1691). In the past, several PCR based methods have been proposed to determine the allelostatus of individual patients from small amounts of blood DNA including PCR followed by restriction fragment length polymorphism detection...
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