Article
Simple and reliable factor V genotyping by PNA-mediated PCR clamping.
Thrombosis and haemostasis - 1 Apr 1998
Behn M, Schuermann M
Abstract excerpt
Resistance to activated protein C (APC resistance) is the most common cause of thrombophilia and linked to a single point mutation in the factor V gene (G-->A transition at nucleotide 1691). In the past, several PCR based methods have been proposed to determine the allelostatus of individual pati...
Topics
- Alleles
- DNA Mutational Analysis
- DNA Primers
- DNA Replication
- Factor V
- Genotype
- Humans
- Magnesium
- Nucleic Acid Denaturation
- Nucleic Acid Synthesis Inhibitors
- Oligopeptides
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Protein C
- Substrate Specificity
- Temperature
- Thrombophilia