Article
Allele-specific PCR amplification of factor V Leiden to identify patients at risk for thromboembolism.
Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine - 1 Jan 1997
Blasczyk R, Wehling J, Ritter M, Neubauer A, Riess H
Abstract excerpt
Resistance to activated protein C is the most common hereditary cause of thrombophilia and is significantly linked to factor V Leiden. We designed primers in order to identify factor V Leiden by allele-specific PCR amplification. Amplification specificity for factor V was ensured by a 3' primer located at the intron 9/exon 10 border of the gene. One sense and two antisense primers were used in two separate primer...
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