p53 mutations and protein expression in primary cultures of normal oral mucosa in smokers and non-smokers.

doi:10.1016/s0964-1955(97)00027-4

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Article

p53 mutations and protein expression in primary cultures of normal oral mucosa in smokers and non-smokers.

Oral oncology - 1 Jul 1997

Colucci S, el-Gehani R, Flint S, Mothersill C

Abstract excerpt

The normal oral mucosa from 77 individuals of known smoking and alcohol-intake history was cultured. After 14-21 days in culture, epithelial cells were stained for p53 expression using immunohistochemistry. PCR-SSCP analysis of the p53 gene was also performed on a random sub-set of these samples...

Topics

Adolescent
Adult
Aged
Cell Culture Techniques
Child
Exons
Female
Gene Expression
Genes, p53
Humans
Immunoenzyme Techniques
Male
Middle Aged
Mouth Mucosa
Mutation
Polymerase Chain Reaction
Polymorphism, Single-Stranded Conformational
Smoking

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