Article
p53 mutations and protein expression in primary cultures of normal oral mucosa in smokers and non-smokers.
Oral oncology - 1 Jul 1997
Colucci S, el-Gehani R, Flint S, Mothersill C
Abstract excerpt
The normal oral mucosa from 77 individuals of known smoking and alcohol-intake history was cultured. After 14-21 days in culture, epithelial cells were stained for p53 expression using immunohistochemistry. PCR-SSCP analysis of the p53 gene was also performed on a random sub-set of these samples (20 non-smokers and 21 smokers). Expression of the stable, non-functional form of p53 protein as detected by the...
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