Article
Probing the structure and function of the estrogen receptor ligand binding domain by analysis of mutants with altered transactivation characteristics.
Molecular and cellular biology - 1 Aug 1997
Eng F C, Lee H S, Ferrara J, Willson T M, White J H
Abstract excerpt
We have developed a genetic screen for the yeast Saccharomyces cerevisiae to isolate estrogen receptor (ER) mutants with altered transactivation characteristics. Use of a "reverse" ER, in which the mutagenized ligand binding domain was placed at the N terminus of the receptor, eliminated the isol...
Topics
- Amino Acid Sequence
- Animals
- COS Cells
- Cloning, Molecular
- Estradiol
- Estrogen Antagonists
- Estrone
- HSP90 Heat-Shock Proteins
- Hydroxyestrones
- Ligands
- Molecular Sequence Data
- Mutation
- Nuclear Proteins
- Polyunsaturated Alkamides
- Receptors, Estrogen
- Saccharomyces cerevisiae
- Sequence Alignment
- Tamoxifen