Article
The normally expressed kappa immunoglobulin light chain gene repertoire and somatic mutations studied by single-sided specific polymerase chain reaction (PCR); frequent occurrence of features often assigned to autoimmunity.
Clinical and experimental immunology - 1 Jul 1997
Juul L, Hougs L, Andersen V, Svejgaard A, Barington T
Abstract excerpt
The expressed human kappa light chain gene repertoire utilized by healthy individuals was studied by two different single-sided specific PCR techniques to avoid bias for certain V genes. A total of 103 rearranged kappa sequences from peripheral blood mononuclear cells from healthy individuals wer...
Topics
- Autoantibodies
- B-Lymphocytes
- Cells, Cultured
- Cloning, Molecular
- DNA, Complementary
- Gene Rearrangement
- Genes, Immunoglobulin
- Genetic Variation
- Humans
- Immunoglobulin kappa-Chains
- Leukocytes, Mononuclear