Article
The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation.
The EMBO journal - 3 Jul 1995
Iost I, Dreyfus M
Abstract excerpt
We have used either Escherichia coli or T7 RNA polymerase to transcribe in E. coli a series of lacZ genes that differ in the nature of their ribosome binding sites (RBS). Each T7 RNA polymerase transcript yields from 15- to 450-fold less beta-galactosidase than its E. coli polymerase counterpart,...
Topics
- Base Sequence
- Binding Sites
- Chromosome Mapping
- DNA-Directed RNA Polymerases
- Enzyme Activation
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Mutation
- Protein Biosynthesis
- RNA, Bacterial
- RNA, Messenger
- Ribosomes
- Viral Proteins
- beta-Galactosidase