Article
Interplay between σ region 3.2 and secondary channel factors during promoter escape by bacterial RNA polymerase.
The Biochemical journal - 1 Dec 2017
Petushkov Ivan, Esyunina Daria, Mekler Vladimir, Severinov Konstantin, Pupov Danil, Kulbachinskiy Andrey
Abstract excerpt
In bacterial RNA polymerase (RNAP), conserved region 3.2 of the σ subunit was proposed to contribute to promoter escape by interacting with the 5'-end of nascent RNA, thus facilitating σ dissociation. RNAP activity during transcription initiation can also be modulated by protein factors that bind within the secondary channel and reach the enzyme active site. To monitor the kinetics of promoter escape in real...
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