Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line.

Journal of virology - 1 Jul 1997

Holzer G W, Falkner F G

Abstract excerpt

The vaccinia virus D4R open reading frame, encoding the essential DNA repair enzyme uracil DNA glycosylase, was expressed in two permanent cell lines, the rabbit kidney cell line RK13 and the human fibroblast cell line 293. The temperature-sensitive vaccinia virus mutant ts4149, which maps within D4R, was able to grow under restrictive conditions in both of these transformed cell lines. Cell clones complemented...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Cell Line
Share to Cloning, Molecular
Share to DNA Repair

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Complementation Test

Share to Mutation

Share to Open Reading Frames

Share to Tumor Cells, Cultured

Share to Viral Proteins

Source

Open in PubMed Open full text

Identifiers

PMID: 9188564
DOI: 10.1128/JVI.71.7.4997-5002.1997
PMCID: PMC191732

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Construction of a vaccinia virus deficient in the essential DNA repair enzyme uracil DNA glycosylase by a complementing cell line.PMID 9188564Read ↗

Vaccinia virus uracil DNA glycosylase has an essential role in DNA synthesis that is independent of its glycosylase activity: catalytic site mutations reduce virulence but not virus replication in cultured cells.Semantic proximity Read ↗

Vaccinia virus encodes an active thymidylate kinase that complements a cdc8 mutant of Saccharomyces cerevisiae.Semantic proximity Read ↗

The vaccinia virus-encoded uracil DNA glycosylase has an essential role in viral DNA replication.Semantic proximity Read ↗

Complementation of a replication-defective mutant of equine herpesvirus type 1 by a cell line expressing the immediate-early protein.Semantic proximity Read ↗

A gene transfer vector-cell line system for complete functional complementation of adenovirus early regions E1 and E4Semantic proximity Read ↗

Complementation of vaccinia virus lacking the double-stranded RNA-binding protein gene E3L by human cytomegalovirus.Semantic proximity Read ↗

Stable expression of the vaccinia virus K1L gene in rabbit cells complements the host range defect of a vaccinia virus mutant.Semantic proximity Read ↗

Analysis of the role of vaccinia virus H7 in virion membrane biogenesis with an H7-deletion mutant.Semantic proximity Read ↗

Highly attenuated modified vaccinia virus Ankara replicates in baby hamster kidney cells, a potential host for virus propagation, but not in various human transformed and primary cells.Semantic proximity Read ↗

Inefficient complementation activity of poliovirus 2C and 3D proteins for rescue of lethal mutations.Semantic proximity Read ↗

A modified screening system for loss-of-function and dominant negative alleles of essential MCMV genes.Semantic proximity Read ↗

IPTG-dependent vaccinia virus: identification of a virus protein enabling virion envelopment by Golgi membrane and egress.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.