Article
Vaccinia virus encodes an active thymidylate kinase that complements a cdc8 mutant of Saccharomyces cerevisiae.
The Journal of biological chemistry - 25 Oct 1991
Hughes S J, Johnston L H, de Carlos A, Smith G L
Abstract excerpt
A vaccinia virus open reading frame (ORF) previously predicted to encode thymidylate kinase (TmpK) is shown to encode an active enzyme. A copy of the ORF, generated by polymerase chain reaction, was cloned into an Escherichia coli inducible expression vector. Cell extracts of E. coli expressing t...
Topics
- Base Sequence
- Blotting, Southern
- DNA, Viral
- Electrophoresis, Polyacrylamide Gel
- Escherichia coli
- Genes, Bacterial
- Genes, Fungal
- Genes, Viral
- Molecular Sequence Data
- Mutation
- Nucleoside-Phosphate Kinase
- Open Reading Frames
- Plasmids
- Precipitin Tests
- Saccharomyces cerevisiae
- Vaccinia virus