Article
IPTG-dependent vaccinia virus: identification of a virus protein enabling virion envelopment by Golgi membrane and egress.
Nucleic acids research - 25 Sept 1990
Rodriguez J F, Smith G L
Abstract excerpt
A novel method has been developed to study the functional roles of individual vaccinia virus gene products that is neither limited by the possible essentiality of the target gene nor by the availability of conditional lethal mutants. The system utilises the E. coli lac repressor protein, the oper...
Topics
- Cell Line
- Escherichia coli
- Gene Expression Regulation, Viral
- Genes, Viral
- Golgi Apparatus
- Humans
- Isopropyl Thiogalactoside
- Microscopy, Electron
- Mutation
- Polymerase Chain Reaction
- Transfection
- Vaccinia virus
- Viral Matrix Proteins
- Virion
- Virus Replication