Mutation of Arg154 to Gly154 in urokinase augments its fibrin-specificity.

doi:10.1080/15216549700201931

Back to search

Article

Mutation of Arg154 to Gly154 in urokinase augments its fibrin-specificity.

Biochemistry and molecular biology international - 1 Apr 1997

Peng G, Ma Z, Kuai L, Zhu D

Abstract excerpt

Rscu-PA and its mutant constructed by in vitro site specific mutagenesis of Arg154 in rscu-PA to Gly154 (mscu-PA) were both expressed in Escherichia coli. After in vitro denaturation and renaturation, the rscu-PA and mscu-PA were purified to homogeneity by Zn2+ selective precipitation, anti-u-PA...

Topics

Arginine
Escherichia coli
Fibrin
Fibrinolysin
Glycine
Kinetics
Mutagenesis, Site-Directed
Mutation
Protein Denaturation
Recombinant Proteins
Sensitivity and Specificity
Substrate Specificity
Urokinase-Type Plasminogen Activator

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.