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Article

Mutation of Arg154 to Gly154 in urokinase augments its fibrin-specificity.

Biochemistry and molecular biology international - 1 Apr 1997

Peng G, Ma Z, Kuai L, Zhu D

Abstract excerpt

Rscu-PA and its mutant constructed by in vitro site specific mutagenesis of Arg154 in rscu-PA to Gly154 (mscu-PA) were both expressed in Escherichia coli. After in vitro denaturation and renaturation, the rscu-PA and mscu-PA were purified to homogeneity by Zn2+ selective precipitation, anti-u-PA IgG-sepharose CL 4B affinity chromatography. After activation by plasmin, the kinetic constants for the resultant...

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Identifiers

PMID: 9137818
DOI: 10.1080/15216549700201931

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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