Characterization of thrombin- and plasmin-resistant mutants of recombinant human single chain urokinase-type plasminogen activator.

doi:10.1093/oxfordjournals.jbchem.a123166

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Article

Characterization of thrombin- and plasmin-resistant mutants of recombinant human single chain urokinase-type plasminogen activator.

Journal of biochemistry - 1 Jul 1990

Eguchi Y, Sakata Y, Matsuda M, Osada H, Numao N, Ohmori M, Kondo K

Abstract excerpt

Recombinant human single-chain urokinase-type plasminogen activator (suc-PA) (SM0: wild type) and its variants resistant to plasmin and/or thrombin (SM1: Lys135 to Gln; SM3: Phe157 to Asp; and SM4: Lys135 to Gln and Phe157 to Asp) have been constructed by site-directed mutagenesis with the aim of...

Topics

Autoradiography
Catalysis
Circular Dichroism
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Fibrinolysin
Fibrinolysis
Humans
Immunodiffusion
Iodine Radioisotopes
Kinetics
Mutation
Plasminogen Activators
Protein Conformation
Recombinant Proteins
Thrombin
Urokinase-Type Plasminogen Activator

Source

Open in PubMed

Identifiers

PMID: 2146258
DOI: 10.1093/oxfordjournals.jbchem.a123166

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2146258-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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