[Application of a gel chromatography renaturing way on low molecular single-chain urokinase mutant(DscuPA-32K)].

Abstract excerpt

A recombinant mutant gene with thrombolytic and antithrombolytic bifunction was expressed in E. coli. Owing to two reasons of high molecular weight and over expression, dscuPA existed in inclusion body form. The protein of inclusion body was inactive protein. In order to obtain active protein, inclusion bodies should be denatured and then renatured. We performed a novel way named gel-chromatography column...

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