Article
Plasminogen activation with single-chain urokinase-type plasminogen activator (scu-PA). Studies with active site mutagenized plasminogen (Ser740----Ala) and plasmin-resistant scu-PA (Lys158----Glu).
The Journal of biological chemistry - 25 Mar 1990
Lijnen H R, Van Hoef B, Nelles L, Collen D
Abstract excerpt
The mechanism of the activation of plasminogen by single-chain urokinase-type plasminogen activator (single-chain u-PA, scu-PA) was studied using rscu-PA-Glu158, a recombinant plasmin-resistant mutant of human scu-PA obtained by site-specific mutagenesis of Lys158 to Glu, and rPlg-Ala740, a recombinant human plasminogen in which the catalytic site is destroyed by mutagenesis of the active-site Ser740 to Ala....
Topics
- Alanine
- Enzyme Activation
- Enzyme Precursors
- Escherichia coli
- Fibrinolysin
- Glutamates
- Glutamic Acid
- Humans
- Kinetics
- Lysine
- Mutation