Article
An enzyme-linked immunosorbent assay for urokinase-type plasminogen activator (u-PA) and mutants and chimeras containing the serine protease domain of u-PA.
Thrombosis and haemostasis - 23 Jan 1992
Declerck P J, Van Keer L, Verstreken M, Collen D
Abstract excerpt
An enzyme-linked immunosorbent assay (ELISA) for quantitation of natural and recombinant plasminogen activators containing the serine protease domain (B-chain) of urokinase-type plasminogen activator (u-PA) was developed, based on two murine monoclonal antibodies, MA-4D1E8 and MA-2L3, raised against u-PA and reacting with non-overlapping epitopes in the B-chain. MA-4D1E8 was coated on microtiter plates and bound...
Topics
- Animals
- Antibodies, Monoclonal
- Chimera
- Cross Reactions
- Enzyme-Linked Immunosorbent Assay
- Epitopes
- Fibrinolysis
- Mutation
- Papio
- Serine Endopeptidases
- Urokinase-Type Plasminogen Activator