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Article

Urokinase mutant with better fibrin-specificity.

Science in China. Series C, Life sciences - 1 Oct 1996

Ma Z, Yu R, Hua Z, Zhu D

Abstract excerpt

A 150-156 amino acids-deleted single-chain urokinase-type plasminogen activator (dscu-PA) and its recombinant wild-type counterpart (rscu-PA) were both expressed in Escherichia coli. After denaturation and renaturation in vitro, the expressed products were both purified to a single silver-stained band by means of IgG affinity chromatography. After activation by plasmin, similar enzymatic constants based on the...

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PMID: 9772355

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Related research

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Research trail→

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Urokinase mutant with better fibrin-specificity.PMID 9772355Read ↗

Mutation of Arg154 to Gly154 in urokinase augments its fibrin-specificity.Semantic proximity Read ↗

Characterization of thrombin- and plasmin-resistant mutants of recombinant human single chain urokinase-type plasminogen activator.Semantic proximity Read ↗

Identification of the mechanism responsible for the increased fibrin specificity of TNK-tissue plasminogen activator relative to tissue plasminogen activator.Semantic proximity Read ↗

[Application of a gel chromatography renaturing way on low molecular single-chain urokinase mutant(DscuPA-32K)].Semantic proximity Read ↗

Making tissue-type plasminogen activator more fibrin specific.Semantic proximity Read ↗

Alteration of serpin specificity by a protein cofactor. Vitronectin endows plasminogen activator inhibitor 1 with thrombin inhibitory properties.Semantic proximity Read ↗

Characterization of domain deletion and/or duplication mutants of a recombinant chimera of tissue-type plasminogen activator and urokinase-type plasminogen activator (rt-PA/u-PA).Semantic proximity Read ↗

Soluble expression of a strong thrombolytic pro-urokinase mutant in Escherichia coli.Semantic proximity Read ↗

Identification of tissue-type plasminogen activator-specific plasminogen activator inhibitor-1 mutants. Evidence that second sites of interaction contribute to target specificity.Semantic proximity Read ↗

Plasminogen activation with single-chain urokinase-type plasminogen activator (scu-PA). Studies with active site mutagenized plasminogen (Ser740----Ala) and plasmin-resistant scu-PA (Lys158----Glu).Semantic proximity Read ↗

Tissue-type plasminogen activator mutants imitating urokinase in the peptide link between kringle and protease domains and at selected sites within the protease domain.Semantic proximity Read ↗

Variants of tissue-type plasminogen activator with substantially enhanced response and selectivity toward fibrin co-factors.Semantic proximity Read ↗

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