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Article

Role of P225 and the C136-C201 disulfide bond in tissue plasminogen activator.

Protein science : a publication of the Protein Society - 1 Aug 1998

Vindigni A, Di Cera E

Abstract excerpt

The protease domain of tissue plasminogen activator (tPA), a key fibrinolytic enzyme, was expressed in Escherichia coli with a yield of 1 mg per liter of media. The recombinant protein was titrated with the Erythrina caraffa trypsin inhibitor (ETI) and characterized in its interaction with plasminogen and the natural inhibitor plasminogen activator inhibitor-1 (PAI-1). Analysis of the catalytic properties of tPA...

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Identifiers

PMID: 10082369
DOI: 10.1002/pro.5560070807
PMCID: PMC2144078

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