Article
Stabilization of yeast artificial chromosome clones in a rad54-3 recombination-deficient host strain.
Nucleic acids research - 15 Mar 1997
Le Y, Dobson M J
Abstract excerpt
The cloning and propagation of large fragments of DNA on yeast artificial chromosomes (YACs) has become a routine and valuable technique in genome analysis. Unfortunately, many YAC clones have been found to undergo rearrangements or deletions during the cloning process. The frequency of transformation-associated alterations and mitotic instability can be reduced in a homologous recombination-deficient yeast host...
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