Article
Stabilization of yeast artificial chromosome clones in a rad54-3 recombination-deficient host strain.
Nucleic acids research - 15 Mar 1997
Le Y, Dobson M J
Abstract excerpt
The cloning and propagation of large fragments of DNA on yeast artificial chromosomes (YACs) has become a routine and valuable technique in genome analysis. Unfortunately, many YAC clones have been found to undergo rearrangements or deletions during the cloning process. The frequency of transform...
Topics
- Chromosomes, Artificial, Yeast
- DNA Helicases
- DNA Repair Enzymes
- DNA, Satellite
- Fungal Proteins
- Gene Library
- Genotype
- Humans
- Mating Factor
- Meiosis
- Mitosis
- Mutagenesis
- Peptides